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JoVE Journal Cancer Research
En 3D spheroid modell som ett mer fysiologiskt system för cancer-associerade fibroblaster differentiering och invasion in vitro-studier
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En 3D spheroid modell som ett mer fysiologiskt system för cancer-associerade fibroblaster differentiering och invasion in vitro-studier

Article DOI: 10.3791/60122-v • 06:27 min • August 8th, 2019
August 8th, 2019 •
1,2, 1
1Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, 2Instituto de Medicina Molecular, Lisbon

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Målet med detta protokoll är att etablera en 3D in vitro-modell för att studera differentiering av cancerassocierade fibroblaster (CAFs) i en tumör bulk-liknande miljö, som kan åtgärdas i olika analyssystem, såsom immunofluorescens, transkriptionella analys och livs cell avbildning.

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Cancer forskning spheroids CAF 3D-modell differentiering Immunofluorescerande färgning qPCR flödescytometri invasion extracellulär matrix gel
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