/
/
En 3D Spheroid model som et mere fysiologisk system for kræft-associeret fibroblaster differentiering og invasion in vitro undersøgelser
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:413D Spheroids as an In Vitro Model for Fibroblast/CAF Differentiation Using Different TGF-β1 Inhibiting Compounds of Cell-matrix Interaction
- 02:02Immunofluorescent Staining of Spheroids
- 04:44Results: A 3D Spheroid Model for Cancer-Associated Fibroblasts Differentiation and Invasion
- 05:37Conclusion
Målet med denne protokol er at etablere en 3D in vitro-model til at studere differentieringen af Cancer-associerede fibroblaster (CAFs) i en tumor bulk-lignende miljø, som kan behandles i forskellige analysesystemer, såsom immunofluorescens, transkriptional analyse og Life Cell Imaging.
