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Chapters
- 00:04Title
- 01:16crRNA Design and Preparation of Cas9/gRNA RNP Complexes
- 02:16Electroporation of RNP Complexes into OCI-AML3 Cells
- 03:12Screening and Selection of Cell Clones with Biallelic Deletions
- 05:51Functional Analyses of Silencer Deletions with Real-time Quantitative RT-PCR
- 07:18Results: RUNX1 Intronic Silencer Deletion
- 08:16Conclusion
Direkte levering af præmonterede Cas9/guide RNA ribonucleoprotein komplekser er et hurtigt og effektivt middel til genomredigering i hæmatopoietiske celler. Her bruger vi denne tilgang til at slette en RUNX1 intronic lyddæmper og undersøge de transkriptionelle svar i OCI-AML3 leukæmiceller.
