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测量自噬和前列腺通量中的日节律
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JoVE Journal Biology
Measuring Diurnal Rhythms in Autophagic and Proteasomal Flux
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测量自噬和前列腺通量中的日节律

DOI:
10.3791/60133-v

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09:18 min

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September 17, 2019

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, ,
1Division of Pulmonary and Critical Care Medicine,Washington University School of Medicine

Chapters

  • 00:04Title
  • 00:40Protease Inhibitor Administration
  • 01:31Tissue Acquisition and Biochemical Fractionation
  • 05:10Western Blotting
  • 06:48Data Analysis
  • 08:06Results: Representative Experimental Flux Results
  • 08:36Conclusion

Summary

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Automatic Translation

我们描述了我们通过自噬和小鼠肝脏蛋白体体来测量蛋白质代谢的生物节律的规程。

Tags

AutophagyProteasomal FluxCircadian RhythmsProtein CatabolismLeupeptinBortezomibHomogenization BufferDounce HomogenizerPost-nuclear SupernatantProtein Concentration

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