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Measuring RAN Peptide Toxicity in C. elegans
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Measuring RAN Peptide Toxicity in C. elegans

Measuring RAN Peptide Toxicity in C. elegans

DOI:
10.3791/61024-v

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10:49 min

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April 30, 2020

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, , ,
1Graduate Program in Cell Biology and Molecular Physiology,University of Pittsburgh, 2Department of Pediatrics,University of Pittsburgh School of Medicine

Chapters

  • 00:04Introduction
  • 01:05Measurement of the Developmental Toxicity of RAN Peptides following RNAi-based Gene Knockdown
  • 05:38Post-developmental RAN Peptide Paralysis Assay
  • 08:02Results: Toxicity of RAN Peptides
  • 10:04Conclusion

Summary

Automatic Translation

Automatic Translation

Repeat-associated non-ATG-dependent translational products are emerging pathogenic features of several repeat expansion-based diseases. The goal of the protocol described is to evaluate toxicity caused by these peptides using behavioral and cellular assays in the model system C. elegans.

Tags

Keywords: RAN PeptidesRepeat Expansion Neurodegenerative DisordersC. Elegans Model SystemRAN Peptide ToxicityMovement AnalysisReproductionIPTGCarbenicillinRNAi Feeding ClonesHypochlorite MethodVideo Speed AnalysisMovement Phenotypes

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