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JoVE Journal
Biochemistry

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Quantification of Coenzyme A in Cells and Tissues
 

Quantification of Coenzyme A in Cells and Tissues

Article DOI: 10.3791/60182-v
September 27th, 2019

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Summary September 27th, 2019

This method describes sample preparation from cultured cells and animal tissues, extraction and derivatization of coenzyme A in the samples, followed by high pressure liquid chromatography for purification and quantification of the derivatized coenzyme A by absorbance or fluorescence detection.

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