Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Our new protocol helps to distinguish and quantify three statuses of tube rings, such as stable microtubules, labile microtubules, and free tubules in animal tissues. This technique is composed of simple steps and can evaluate the structural stability of tubing, which cannot be detected by quantification of tubing post translational modification. This method is essential for the research and development of therapeutic ways for diseases where microtubule stability is critical, such as Alzheimer's disease and cancers.

Start the procedure by preparing the lab wear for tissue dissection. Fill a box with crushed ice and place two Petri dishes on it. Fill one dish with ice cold phosphate buffer solution or PBS for transient wash and storage of dissected tissues.