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Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
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Biochemistry
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JoVE Journal Biochemistry
Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo

Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo

DOI:
10.3791/63681-v

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10:35 min

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April 06, 2022

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, , , , ,
1Department of Human Sciences,The Ohio State University

Chapters

  • 00:04Introduction
  • 01:043T3‐L1 Cell Culture and Maintenance
  • 01:49Starvation of 3T3‐L1 Cells
  • 02:22Preparation of FD‐Glucose Solutions with Different Concentrations
  • 04:19Treating Starved 3T3‐L1 Cells
  • 05:03Extracellular and Intercellular Measurements for Stimulated 3T3‐L1 Cells
  • 06:00Protein‐Based Normalization of Intercellular Glucose Uptake
  • 06:35Ex Vivo Measurement of Extracellular FD‐Glucose Depletion in Organs
  • 07:59Results: Extracellular FD‐Glucose Correlation with Intracellular Uptake and Kinetics of Extracellular FD‐Glucose Depletion
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

Extracellular depletion of fluorescently labeled glucose correlates with glucose uptake and could be used for high-throughput screening of glucose uptake in excised organs and cell cultures.

Tags

Keywords: Glucose UptakeExtracellular Glucose DepletionHigh Throughput AssaysKineticsCell Culture3T3-L1 FibroblastsFastingStimulationInsulinGlucose ConcentrationMetabolic DiseasesDiabetesCancerObesityCentral Nervous System Diseases

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