Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo

Glucose uptake is the key to understanding health and disease. As glucose plays a pivotal role in metabolic and structural demands in the human body, as well as playing a vital role in regulation. The extracellular measurement of glucose allows for the development of high throughput assays for kinetics of glucose uptake in cells, tissues, and organs of different genetic background in response to nutrients or drugs.

This assay would be a valuable tool for the discovery of therapeutics and nutrition for diabetes, cancer, degenerative diseases of the central nervous system, obesity, and all the diseases of glucose and metabolism. The assay needs an adjustment of time for starvation and stimulation that should be based on metabolic specifics for different tissues and cell cultures. Culture 3T3-L1 mouse fibroblasts by plating 10 to the 6 cells, diluted in 20 milliliters of medium 1 in 296 well plates.