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细菌肽显示用于选择新型生物仿生酶
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
DOI:

10:43 min

October 03, 2019

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Chapters

  • 00:04Title
  • 00:29Insertion of Peptide Coding Sequencing Sequence in pBAD BirA-eCPX-AP
  • 01:32Generation of a BirA Library
  • 03:16Selection of Bacteria Expressing Biotinylated Peptide
  • 07:18Quantification of Enrichment
  • 08:26Results: Model Selection with pBAD-BirA-eCPX-AP and pBAD-BirA-eCPX-AP(K10A)
  • 10:00Conclusion

Summary

Automatic Translation

在这里,我们提出一种方法,以选择大肠杆菌生物蛋白的倍酶BirA的新变种,生物异化特定靶肽。该协议描述了用于靶肽的细菌显示的质粒的构造、BirA库的生成、BirA变体的选择和表征。

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