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Etablering og karakterisering av små tarm Nevroendokrine tumor Spheroids

Article DOI: 10.3791/60303-v • 09:43 min • October 14th, 2019
October 14th, 2019 •
1, 1, 2, 3, 3, 1
1Department of Surgery, University of Iowa Carver College of Medicine, 2High Throughput Screening Facility, University of Iowa Carver College of Medicine, 3Department of Pathology, University of Iowa Carver College of Medicine

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Nevroendokrine svulster (NETs) stammer fra Nevroendokrine celler av Neural Crest. De er langsom voksing og utfordrende å kulturen. Vi presenterer en alternativ strategi for å vokse garn fra tynntarmen ved å dyrking dem som spheroids. Disse spheroids har liten tarmen NET markører og kan brukes til narkotika testing.

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Kreftforskning små tarm Nevroendokrine svulster spheroids synaptophysin kromogranin A SSTR2 immunofluorescence immunhistokjemi ekstracellulære matrise Rapamycin
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