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小肠神经内分泌肿瘤类球体的建立与特征
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Cancer Research
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JoVE Journal Cancer Research
Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids
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小肠神经内分泌肿瘤类球体的建立与特征

DOI:
10.3791/60303-v

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09:43 min

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October 14, 2019

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, , , , ,
1Department of Surgery,University of Iowa Carver College of Medicine, 2High Throughput Screening Facility,University of Iowa Carver College of Medicine, 3Department of Pathology,University of Iowa Carver College of Medicine

Chapters

  • 00:04Title
  • 00:44Small Bowel Neuroendocrine Tumor (SBNET) Collection and Cell Dissociation
  • 02:10Culture of SBNET as Tumor Spheroids in ECM
  • 04:02Characterization of SBNET Spheroids by Immunofluorescence
  • 05:50Splitting SBNET Spheroids
  • 07:23Results: Immunofluorescence Imaging and Drug Testing of SBNET Spheroids
  • 09:02Conclusion

Summary

Automatic Translation

Automatic Translation

神经内分泌肿瘤（NETs）来自神经峰的神经内分泌细胞。它们生长缓慢，对文化充满挑战。我们提出了一个替代策略，通过培养它们作为球体，从小肠中种植NET。这些球体有小肠NET标记，可用于药物测试。

Tags

Small Bowel Neuroendocrine TumorSBNET3D CultureSpheroidsCell LineNeuroendocrine TumorCell IsolationCell DigestionCell CountingExtracellular Matrix

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