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小肠神经内分泌肿瘤类球体的建立与特征
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids
DOI:

09:43 min

October 14, 2019

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Chapters

  • 00:04Title
  • 00:44Small Bowel Neuroendocrine Tumor (SBNET) Collection and Cell Dissociation
  • 02:10Culture of SBNET as Tumor Spheroids in ECM
  • 04:02Characterization of SBNET Spheroids by Immunofluorescence
  • 05:50Splitting SBNET Spheroids
  • 07:23Results: Immunofluorescence Imaging and Drug Testing of SBNET Spheroids
  • 09:02Conclusion

Summary

Automatic Translation

神经内分泌肿瘤(NETs)来自神经峰的神经内分泌细胞。它们生长缓慢,对文化充满挑战。我们提出了一个替代策略,通过培养它们作为球体,从小肠中种植NET。这些球体有小肠NET标记,可用于药物测试。

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