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通过不同的均质化方法从小鼠大脑/脊髓中检索到的多种细胞类型的同步流细胞测量特征
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JoVE Journal Medicine
Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods
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通过不同的均质化方法从小鼠大脑/脊髓中检索到的多种细胞类型的同步流细胞测量特征

DOI:
10.3791/60335-v

•

10:24 min

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November 19, 2019

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, , ,
1Dana-Farber/Boston Children’s Cancer and Blood Disorders Center, 2Harvard Medical School

Chapters

  • 00:04Title
  • 00:49Enzymatic Brain and Spinal Cord Digestion
  • 03:58Mechanical Brain and Spinal Cord Homogenization
  • 05:00Debris Removal
  • 06:19Flow Cytometric Cell Staining
  • 07:48Results: Representative Relative Proportions of Different Cell Types Retrieved from the CNS According to the Tissue Homogenization Method
  • 09:38Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出了一种流式细胞测定方法，用于识别从小鼠大脑或脊髓中检索到的不同细胞类型。该方法可用于分离或描述神经退行性疾病中的纯细胞群，或量化细胞靶向在体内给病毒载体或纳米粒子的靶向范围。

Tags

Flow CytometryCell CharacterizationMouse BrainSpinal CordCell IsolationGene DeliveryDrug DeliveryNeurodegenerative Disorders

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