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1Department of Pharmacology and Toxicology,University of Louisville, 2Hepatobiology and Toxicology Program,University of Louisville, 3Department of Physiology and Pharmacology,West Virginia University, 4Center for Inhalation Toxicology,West Virginia University, 5Department of Pharmacology and Chemical Biology,University of Pittsburgh, 6Pittsburgh Liver Research Center,University of Pittsburgh, 7Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition,University of Pittsburgh
Chapters
- 00:04Title
- 00:42Experimental Setup and Purified Experimental Diet Acclimatization
- 01:21Vinyl Chloride (VC) Inhalation Exposure System Setup
- 02:17Pre-Exposure Setup
- 03:21Exposure Experiment and VC Concentration Validation
- 06:12Post-Exposure
- 07:01Results: Representative Effects of VC on the Enhancement of Diet-Induced Liver Injury
- 08:29Conclusion
The goal of this protocol was to develop a murine model of low-level toxicant exposure that does not cause overt liver injury but rather exacerbates pre-existing liver damage. This paradigm better recapitulates human exposure and the subtle changes that occur upon exposure to toxicant concentrations that are considered safe.
