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JoVE Journal Bioengineering
Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
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Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Article DOI: 10.3791/60388-v • 09:11 min • March 22nd, 2020
March 22nd, 2020 •
1, 1, 2, 3, 1,4, 3, 1
1Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, 2Department of Chemical Physics and Optics, Faculty of Mathematics and Physics, Charles University, 3Department of Cell Biology, Faculty of Science, Charles University, 4First Faculty of Medicine, Charles University

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Summary
Automatic Translation

A reliable and easily reproducible method for preparation of functionalizable, near-infrared emitting photoluminescent gold nanoclusters and their direct detection inside HeLa cells by flow cytometry and confocal laser scanning microscopy is described.

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Gold Nanoclusters Near-infrared Emitting Synthesis Functionalizable Photoluminescent Ligand Attachment Amine-functionalized Ligand Coil Stability In Vitro Detection Biosensing Solubling Bio-imaging Flow Cytometry Microscopic Part Thioctic Acid Sodium Hydroxide Ultrapure Water Hydrogen Tetrachloraurate Sodium Borohydride Reaction Mixture Purification Ultrafiltration
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