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JoVE Journal Bioengineering
Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
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Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Article DOI: 10.3791/63857-v • 05:26 min • April 13th, 2022
April 13th, 2022 •
1, 2, 2, 1
1Department of Chemical and Biological Engineering, Colorado School of Mines, 2Department of Orthopedics, University of Colorado Anschutz Medical Campus

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Summary
Automatic Translation

The present protocol describes a non-emulsion-based method for the fabrication of chitosan-genipin microgels. The size of these microgels can be precisely controlled, and they can display pH-dependent swelling, degrade in vivo, and be loaded with therapeutic molecules that release over time in a sustained manner, making them highly relevant for tissue engineering applications.

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Fabrication Size-controlled Emulsion-free Chitosan Microgels Tissue Engineering Drug Delivery Toxic Solvents Biocompatible Clinical Setting Biomaterial Strategy Growth Plate Injuries Regenerative Medicine Applications Injectable Biodegradable Scaffold System Sustained Drug Release Acetic Acid Purified Chitosan Solution Luer Lock Syringe Genipin Solution Petri Dish
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