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Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
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Chapters
- 00:05Introduction
- 01:02Formation of Chitosan Hydrogel
- 01:59Filtration of the Prepared Chitosan Microgel
- 02:49Purification of the Filtered Microgels
- 03:52Results: pH-Dependent Swelling Behavior of the Microgels and Effects of Microgels on Physical Injury
- 04:45Conclusion
The present protocol describes a non-emulsion-based method for the fabrication of chitosan-genipin microgels. The size of these microgels can be precisely controlled, and they can display pH-dependent swelling, degrade in vivo, and be loaded with therapeutic molecules that release over time in a sustained manner, making them highly relevant for tissue engineering applications.
