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Bioengineering
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近赤外線発光金ナノクラスターの生物応用のための合成
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
Please note that all translations are automatically generated.
Click here for the English version.
近赤外線発光金ナノクラスターの生物応用のための合成
DOI:
10.3791/60388-v
•
09:11 min
•
March 22, 2020
•
Goutam Pramanik
,
Alena Keprova
,
Jan Valenta
,
Vaclav Bocan
,
Klaudia Kvaková
4
,
Lenka Libusova
,
Petr Cigler
1
Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences
,
2
Department of Chemical Physics and Optics, Faculty of Mathematics and Physics
,
Charles University
,
3
Department of Cell Biology, Faculty of Science
,
Charles University
,
4
First Faculty of Medicine
,
Charles University
Chapters
00:05
Introduction
01:10
Preparation of Near-infrared Emitting AuNCs
02:13
Conjugation of TPP on the Surface of 1
02:55
AuNC Internalization into HeLa Cells
03:55
Flow Cytometry Analysis
05:26
CLSM Imaging of Live HeLa Cells Labeled with 2
07:15
Results: Gold Nanoclusters inside HeLa Cells
08:23
Conclusion
Summary
Automatic Translation
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Automatic Translation
可動性の高い、近赤外線発光光発光金ナノクラスターの調製法と、フローサイトメトリーおよび共焦点レーザー走査顕微鏡によるHeLa細胞内での直接検出の信頼性と再現性の高い方法について説明する。
Tags
Gold Nanoclusters
Near-infrared Emitting
Photoluminescent
Thiol-functionalized Ligand
Amine-functionalized Ligand
Biosensing
Bio-imaging
Ultrafiltration
Polyethylene Glycol
EDC HCL
Cell Culture
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