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Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
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Bioengineering
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JoVE Journal Bioengineering
Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

DOI:
10.3791/60388-v

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09:11 min

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March 22, 2020

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, , , , , ,
1Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, 2Department of Chemical Physics and Optics, Faculty of Mathematics and Physics,Charles University, 3Department of Cell Biology, Faculty of Science,Charles University, 4First Faculty of Medicine,Charles University

Chapters

  • 00:05Introduction
  • 01:10Preparation of Near-infrared Emitting AuNCs
  • 02:13Conjugation of TPP on the Surface of 1
  • 02:55AuNC Internalization into HeLa Cells
  • 03:55Flow Cytometry Analysis
  • 05:26CLSM Imaging of Live HeLa Cells Labeled with 2
  • 07:15Results: Gold Nanoclusters inside HeLa Cells
  • 08:23Conclusion

Summary

Automatic Translation

Automatic Translation

A reliable and easily reproducible method for preparation of functionalizable, near-infrared emitting photoluminescent gold nanoclusters and their direct detection inside HeLa cells by flow cytometry and confocal laser scanning microscopy is described.

Tags

Keywords: Gold NanoclustersNear-infrared EmittingPhotoluminescentThiol-functionalized LigandAmine-functionalized LigandBiosensingBio-imagingUltrafiltrationPolyethylene GlycolEDC HCLCell Culture

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