/
/
Stereologisk estimering av kolinerge fiberlengde i nucleus basalis av Meynert av mushjernen
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Laboratory for Alzheimer’s Disease and Aging Research,Kansas City Veterans Affairs Medical Center, 2Department of Neurology,University of Kansas Medical Center, 3Department of Molecular and Integrative Physiology,University of Kansas Medical Center, 4The University of Kansas Alzheimer’s Disease Center
Chapters
- 00:04Introduction
- 01:09Perfusion and Tissue Processing
- 02:45Systematic Section Selection
- 03:19Immunohistochemistry (IHC)
- 04:46Stereology
- 08:10Results: Representative Mouse Brain Cholinergic Fiber Length Estimation
- 08:38Conclusion
Neuronal fiber lengde innenfor en tredimensjonal struktur av en hjerneregion er en pålitelig parameter for å kvantifisere spesifikk nevronal strukturell integritet eller degenerasjon. Denne artikkelen beskriver en stereologisk kvantifiseringsmetode for å måle kolinerge fiberlengde innenfor kjernen basalis av Meynert hos mus som et eksempel.
Tags
Keywords:
Stereological EstimationCholinergic Fiber LengthNucleus Basalis Of MeynertMouse BrainNeurodegenerationNeuronal Fiber LossCell Body LossStereological QuantificationUnbiased FractionationLine-like StructuresNeuronal FibersTranscardial PerfusionDPBSPFASucroseCryotomeSectioningImmunohistochemical LabelingNormal Bovine SerumTriton X-100Tris-buffered Saline
