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JoVE Journal Cancer Research
In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System
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In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System

Article DOI: 10.3791/60410-v • 05:45 min • January 9th, 2020
January 9th, 2020 •
*1,2, *1,2, 3, 2,4, 1,2
1The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben-Gurion University of the Negev, 2Faculty of Health Sciences, Ben-Gurion University of the Negev, 3Human Oncology & Pathogenesis Program (HOPP), Memorial Sloan Kettering Cancer Center, 4Institute of Pathology, Barzilai University Medical Center
* These authors contributed equally

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Le développement de modèles murins avec des gènes spécifiques mutés dans les patients atteints de cancer de la tête et du cou est nécessaire pour la compréhension de la néoplasie. Ici, nous présentons un protocole pour la transformation in vitro des cellules primaires de langue murine utilisant un système adeno-associé de virus-Cas9 pour produire des lignes de cellules murines de HNC avec des altérations génomiques spécifiques.

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Recherche sur le cancer numéro 155 cancer de la tête et du cou modèles de cancer de la murine édition génomique CRISPR vecteur de virus adéno-associé transformation cellulaire in vitro lignée de cellules tumorales
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