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Journal / Cancer Research / In vitro etablering af en genetisk manipuleret…
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Cancer Research

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JoVE Journal Cancer Research
In vitro etablering af en genetisk manipuleret murine hoved og hals Cancer cellelinje ved hjælp af en adeno-associeret virus-Cas9 system
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In vitro etablering af en genetisk manipuleret murine hoved og hals Cancer cellelinje ved hjælp af en adeno-associeret virus-Cas9 system

Article DOI: 10.3791/60410-v • 05:45 min • January 9th, 2020
January 9th, 2020 •
*1,2, *1,2, 3, 2,4, 1,2
1The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben-Gurion University of the Negev, 2Faculty of Health Sciences, Ben-Gurion University of the Negev, 3Human Oncology & Pathogenesis Program (HOPP), Memorial Sloan Kettering Cancer Center, 4Institute of Pathology, Barzilai University Medical Center
* These authors contributed equally

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Udvikling af murine modeller med specifikke gener muteret i hoved og halscancer patienter er nødvendig for forståelsen af neoplasi. Her præsenterer vi en protokol for in vitro-omdannelse af primære murine tunge celler ved hjælp af en adeno-associeret virus-Cas9 system til at generere murine HNC cellelinjer med specifikke genomiske ændringer.

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Kræftforskning hoved og hals kræft murine kræft modeller genomredigering CRISPR adeno-associeret virus vektor in vitro celletransformation tumor cellelinje
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