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Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis
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1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital,Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital,Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital,Central South University, 4National Clinical Research Center for Geriatric Disorders, Xiangya Hospital,Central South University, 5Department of Obstetrics and Gynecology, Xiangya Hospital,Central South University
Chapters
- 00:04Title
- 01:49Preparation of Mitochondria from Human Ovarian Cancer Tissues
- 05:00Preparation of Mitochondria from Human Control Ovarian Tissues
- 08:44Results: Analysis of the Prepared Mitochondrial Samples
- 10:42Conclusion
This article presents a protocol of differential-speed centrifugation in combination with density gradient centrifugation to separate mitochondria from human ovarian cancer tissues and control ovarian tissues for quantitative proteomics analysis, resulting in a high-quality mitochondrial sample and high-throughput and high-reproducibility quantitative proteomics analysis of a human ovarian cancer mitochondrial proteome.
