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Utarbeidelse av mitokondrier fra eggstokkene Cancer vev og kontroll eggstokkene vev for kvantitativ Proteomikk analyse
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1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital,Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital,Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital,Central South University, 4National Clinical Research Center for Geriatric Disorders, Xiangya Hospital,Central South University, 5Department of Obstetrics and Gynecology, Xiangya Hospital,Central South University
Chapters
- 00:04Title
- 01:49Preparation of Mitochondria from Human Ovarian Cancer Tissues
- 05:00Preparation of Mitochondria from Human Control Ovarian Tissues
- 08:44Results: Analysis of the Prepared Mitochondrial Samples
- 10:42Conclusion
Denne artikkelen presenterer en protokoll av differensial-Speed sentrifugering i kombinasjon med tetthet gradient sentrifugering å skille mitokondrier fra humant eggstokkene kreft vev og kontroll eggstokkene vev for kvantitativ Proteomikk analyse, resulterer i en høy kvalitet mitokondrie prøven og høy-gjennomstrømning og høy reproduserbarhet kvantitativ Proteomikk analyse av en menneskelig kreft i eggstokkene mitokondrie proteom.
