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使用酵母毒性和抑制器屏幕识别细菌效应蛋白的宿主通路
JoVE Journal
Genetics
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JoVE Journal Genetics
Identification of Host Pathways Targeted by Bacterial Effector Proteins using Yeast Toxicity and Suppressor Screens
DOI:

07:40 min

October 25, 2019

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Chapters

  • 00:04Title
  • 00:46Toxicity Testing of the Protein of Interest
  • 01:53Transformation of Toxic Yeast with the Yeast Genomic Library
  • 05:44Results: Yeast Suppressor Screen
  • 06:48Conclusion

Summary

Automatic Translation

细菌病原体将蛋白质分泌到宿主中,以关键生物过程为目标。识别细菌效应蛋白所针对的宿主通路是解决分子发病机制的关键。这里,描述了一种使用改性酵母抑制剂和毒性筛分的方法,以阐明有毒细菌效应蛋白所针对的宿主通路。

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