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使用消耗法对溶液分散无机纳米颗粒进行短肽吸附研究

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Chemistry
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JoVE Journal Chemistry

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

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使用消耗法对溶液分散无机纳米颗粒进行短肽吸附研究

DOI:

10.3791/60526-v

•

09:43 min

•

April 11, 2020

•

Elena Korina, Sergei Naifert, Roman Morozov, Vladimir Potemkin², Oleg Bol’shakov

¹Nanotechnology Education and Research Center,South Ural State University, ²Laboratory of Computational Modelling of Drugs,South Ural State University

Chapters

00:00Introduction
00:58Preparation of 16 mM Dipeptide Stock Solution
02:18Preparation of Peptide Dilutions
03:25Preparation of Titania Sole
04:26Mixing and Thermostating
05:32Filtration of Thermostated Samples
06:10Preparation of Derivatization Solutions
06:58Derivatization
07:37HPLC Analysis
07:58Results
09:05Conclusion

Summary

Automatic Translation

理解生物分子-无机固相相互作用的第一步是揭示基本的物理化学常数，可以通过建立吸附等体来评估这些常数。液相的吸附受受动力学、表面容量、pH 和竞争吸附的限制，在设置吸附实验之前，应谨慎考虑这些。

Tags

Keywords: Peptide Adsorption Inorganic Nanoparticles Depletion Method Adsorption Isotherms Dipeptide Adsorption Titanium Dioxide PH Adjustment MES Buffer Solution Preparation Concentration Dilution

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