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Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia
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JoVE Journal Neuroscience
Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia

Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia

DOI:
10.3791/60548-v

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07:32 min

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February 09, 2020

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, , , ,
1Department of Biomedical Sciences,University of Illinois College of Medicine Rockford, 2Department of Bioengineering,University of Illinois at Chicago, 3MD Program,University of Illinois College of Medicine Rockford

Chapters

  • 00:04Introduction
  • 00:50Telencephalic Glutamatergic Neuron Generation
  • 01:49Time-Lapse Imaging of Mitochondrial Transport Along Telencephalic Glutamatergic Neuron Axons
  • 02:44Cortical Neuron Mitochondrial Transport Analysis
  • 04:17Cortical Neuron Mitochondrial Morphology Analysis
  • 05:32Results: Representative Mitochondrial Transport and Morphology Analyses
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

Impaired mitochondrial transport and morphology are involved in various neurodegenerative diseases. The presented protocol uses induced pluripotent stem cell-derived forebrain neurons to assess mitochondrial transport and morphology in hereditary spastic paraplegia. This protocol allows characterization of mitochondrial trafficking along axons and analysis of their morphology, which will facilitate the study of neurodegenerative disease.

Tags

Keywords: Mitochondrial TransportMitochondrial MorphologyHuman Induced Pluripotent Stem Cell-derived NeuronsHereditary Spastic ParaplegiaAxonal DegenerationMitochondrial DynamicsNeurological DiseasesLive Cell ImagingMitochondrial TraffickingTherapeutic TargetsNeurodegenerative DiseasesNeurosphere DifferentiationAxon IdentificationMitochondrial StainingTime-lapse ImagingImageJ Analysis

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