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Neuroscience
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从产后小鼠小脑中纯化Promin-1
+
干细胞
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Purification of Prominin-1
+
Stem Cells from Postnatal Mouse Cerebellum
Please note that all translations are automatically generated.
Click here for the English version.
从产后小鼠小脑中纯化Promin-1
+
干细胞
DOI:
10.3791/60554-v
•
07:04 min
•
April 12, 2020
•
Chandrakanth Reddy Edamakanti
,
Puneet Opal
2
1
Davee Department of Neurology
,
Northwestern University Feinberg School of Medicine Chicago
,
2
Department of Cell and Molecular Biology
,
Northwestern University Feinberg School of Medicine Chicago
Chapters
00:05
Introduction
00:53
Cerebellum Dissection and Cell Suspension Preparation
02:34
Immunolabeling of Stem Cells
03:44
Magnetic Column Preparation and Cell Sorting
04:22
Passaging of Neurospheres and Differentiation
05:27
Results: Prominin-1 Stem Cells Isolated from Postnatal Cerebellum
06:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里演示的是一种高效且经济高效的方法,用于从产后小鼠小脑中纯化、培养和分化白质干细胞。
Tags
Prominin-1
Stem Cells
Cerebellum
Postnatal
FACS
Papain
DNAse
Centrifugation
Cell Strainer
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