Nestin-expressing progenitors are a newly identified population of neuronal progenitors in the developing cerebellum. Using the microdissection technique presented here in combination with fluorescent-activated cell sorting, this cell population can be purified with no contamination from other cerebellar regions and can be cultured for further studies.
Yuelling, L. W., Du, F., Li, P., Muradimova, R. E., Yang, Z. Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection. J. Vis. Exp. (91), e52034, doi:10.3791/52034 (2014).