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Neuroscience
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プロミニニン
-1+
出生後マウス小脳由来幹細胞の精製
JoVE Journal
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JoVE Journal
Neuroscience
Purification of Prominin-1
+
Stem Cells from Postnatal Mouse Cerebellum
Please note that all translations are automatically generated.
Click here for the English version.
プロミニニン
-1+
出生後マウス小脳由来幹細胞の精製
DOI:
10.3791/60554-v
•
07:04 min
•
April 12, 2020
•
Chandrakanth Reddy Edamakanti
,
Puneet Opal
2
1
Davee Department of Neurology
,
Northwestern University Feinberg School of Medicine Chicago
,
2
Department of Cell and Molecular Biology
,
Northwestern University Feinberg School of Medicine Chicago
Chapters
00:05
Introduction
00:53
Cerebellum Dissection and Cell Suspension Preparation
02:34
Immunolabeling of Stem Cells
03:44
Magnetic Column Preparation and Cell Sorting
04:22
Passaging of Neurospheres and Differentiation
05:27
Results: Prominin-1 Stem Cells Isolated from Postnatal Cerebellum
06:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここで実証されているのが、白物質幹細胞を出生後マウス小脳から精製、培養、分化するための効率的で費用対効果の高い方法です。
Tags
Prominin-1
Stem Cells
Cerebellum
Postnatal
FACS
Papain
DNAse
Centrifugation
Cell Strainer
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