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In Vivo Mouse Model of Spinal Implant Infection
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JoVE Journal Medicine
In Vivo Mouse Model of Spinal Implant Infection

In Vivo Mouse Model of Spinal Implant Infection

DOI:
10.3791/60560-v

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08:03 min

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June 23, 2020

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, , , , , , , , , , , , , , , , , , , ,
1Department of Orthopaedic Surgery,University of California Los Angeles, 2David Geffen School of Medicine,University of California Los Angeles, 3University of South Carolina School of Medicine,University of South Carolina

Chapters

  • 00:04Introduction
  • 00:39S. aureus Preparation
  • 01:57Mouse Implant Placement and Inoculation
  • 04:09Longitudinal In Vivo Bioluminescence Imaging (BLI)
  • 04:46Implant‐Adherent and Surrounding Tissue Bacteria Quantification
  • 06:16Results: Representative Bacterial Burden Quantification and Analysis
  • 07:30Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol describes a novel in vivo mouse model of spinal implant infection where a stainless-steel k-wire implant is infected with bioluminescent Staphylococcus aureus Xen36. Bacterial burden is monitored longitudinally with bioluminescent imaging and confirmed with colony forming unit counts after euthanasia.

Tags

Keywords: Spinal Implant InfectionMouse ModelS. AureusIn VivoBacterial BurdenPostoperative InfectionSpinal SurgeryHost FactorsImplant FactorsTherapeutic FactorsLongitudinal Study

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