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Journal / Genetics / Kombinera Laser Capture Microdissection och…
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Genetics

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JoVE Journal Genetics
Kombinera Laser Capture Microdissection och Microfluidic qPCR att analysera transkriptionella profiler av enskilda celler
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Kombinera Laser Capture Microdissection och Microfluidic qPCR att analysera transkriptionella profiler av enskilda celler: A Systems Biology strategi för opioidberoende

Article DOI: 10.3791/60612-v • 09:54 min • March 8th, 2020
March 8th, 2020 •
1,2, 3, 1, 3, 1
1Daniel Baugh Institute for Functional Genomics and Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, 2Sidney Kimmel Medical College, Thomas Jefferson University, 3Department of Pharmacology & Physiology, Drexel University College of Medicine

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Detta protokoll förklarar hur man samlar in enstaka nervceller, mikroglia och astrocyter från den centrala kärnan i amygdala med hög noggrannhet och anatomisk specificitet med hjälp av laser fånga microdissection. Dessutom förklarar vi vår användning av microfluidic RT-qPCR för att mäta en delmängd av transkriptomen i dessa celler.

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Genetik encelliga genuttryck laserfångande mikrodissektion mikrofluidisk qPCR opioidberoende anatomisk specificitet amygdala gutmikrobiomet glia
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