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膜和细胞壁选择性荧光染料在丝状真菌活细胞成像中的应用
JoVE Journal
Biology
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JoVE Journal
Biology
Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi
Please note that all translations are automatically generated.
Click here for the English version.
膜和细胞壁选择性荧光染料在丝状真菌活细胞成像中的应用
DOI:
10.3791/60613-v
•
07:44 min
•
November 28, 2019
•
Alexander Lichius
,
Susanne Zeilinger
1
Department of Microbiology
,
University of Innsbruck
Chapters
00:04
Title
01:06
Preparation of Fungal Samples
01:40
Sample Preparation for Microscopy
02:57
Live-cell Microscopy
04:16
Results: Live-Cell Imaging of Filamentous Fungi
06:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
生命荧光染料是现代真菌细胞生物学中活细胞成像分析的重要工具。本文详细介绍了已建立的和鲜为人知的荧光染料在丝状真菌中跟踪血浆膜动力学、内膜/外细胞化和细胞壁形态发生中的应用。
Tags
Membrane Dyes
Cell Wall Dyes
Live-cell Imaging
Filamentous Fungi
Fungal Cells
Organelle Dynamics
Vital Staining
Image-guided Diagnosis
Aspergillosis
Drug Uptake Dynamics
Sample Preparation
Microscopy
Image Acquisition Settings
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