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JoVE Journal
Biology

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Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
 

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Article DOI: 10.3791/945
November 26th, 2008

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Summary November 26th, 2008

A simple and specific method was demonstrated for fluorescent labeling and enhanced detection of cell surface proteins without a fractionation step. Differential abundance in cell surface proteins was analyzed using two-dimensional (2-D) electrophoresis and Ettan™ DIGE technology.

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