Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Selective Labelling of Cell-surface Proteins…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
Measuring the Induced Membrane Voltage with Di-8-ANEPPS
Journal (Biology)
Measuring the Induced Membrane Voltage with Di-8-ANEPPS
The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example
Journal (Biology)
The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example
 

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Article DOI: 10.3791/945 • 14:43 min • November 26th, 2008
November 26th, 2008 •
1, 1, 1, 1
1Research and Development, GE Healthcare Bio-Sciences AB

Chapters

Summary
Automatic Translation

A simple and specific method was demonstrated for fluorescent labeling and enhanced detection of cell surface proteins without a fractionation step. Differential abundance in cell surface proteins was analyzed using two-dimensional (2-D) electrophoresis and Ettan™ DIGE technology.

Tags

Selective Labelling Cell-surface Proteins CyDye DIGE Fluor Minimal Dyes Surface Proteins Intracellular Signaling Environmental Adaptation Drug Treatment Disease Pathogenesis Pathology Protein-protein Interactions Sensitive Methods Reliable Methods Cell Surface Proteins Detection 2-D Electrophoresis Biomarkers Detection Complex Protein Samples Differential Changes Low Abundance Proteins Fractionation Enrichment Hydrophobic Nature High Molecular Weight Proteins Intact Cells Protocol Specific Labeling CyDye DIGE Fluor Minimal Dyes
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter