Journal
/
Biology
/
Anvendelse af membran og cellevæg selektive fluorescerende farvestoffer til levende celle billeder af filamentøse svampe
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi

Anvendelse af membran og cellevæg selektive fluorescerende farvestoffer til levende celle billeder af filamentøse svampe

DOI:
10.3791/60613-v

•

07:44 min

•

November 28, 2019

•

,
1Department of Microbiology,University of Innsbruck

Chapters

  • 00:04Title
  • 01:06Preparation of Fungal Samples
  • 01:40Sample Preparation for Microscopy
  • 02:57Live-cell Microscopy
  • 04:16Results: Live-Cell Imaging of Filamentous Fungi
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

Vitale fluorescerende farvestoffer er essentielle værktøjer til analyser af levende celler i moderne svampe cellebiologi. Dette papir beskriver anvendelsen af etablerede og mindre kendte fluorescerende farvestoffer til sporing af plasma membran dynamik, endo-/exocytose og cellevæg morfogenesis i filamentøse svampe.

Tags

Membrane DyesCell Wall DyesLive-cell ImagingFilamentous FungiFungal CellsOrganelle DynamicsVital StainingImage-guided DiagnosisAspergillosisDrug Uptake DynamicsSample PreparationMicroscopyImage Acquisition Settings

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Levende Cell Imaging af Early Autophagy begivenheder: Omegasomes and Beyond

Intravital mikroskopi af Imaging Subcellulære Strukturer Live mus, der udtrykker fluorescerende proteiner

G Protein-selektive GPCR Konformationer målt med FRET sensorer i en Live Cell Suspension Fluorometer Assay

Undersøgelse af Protein Kvalitetskontrol System af<em> D. discoideum</em> Brug Temperatur-kontrollerede Live-Cell Imaging

Levende cellebilleddannelse af blodpladegravulation og sekretion under strømning

Dynamisk cellebilledbehandling i superopløsning af undercellulære strukturer

Read Article