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Journal
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Biochemistry
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使用碘电磷诱导红血球中的红血球
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore
Please note that all translations are automatically generated.
Click here for the English version.
使用碘电磷诱导红血球中的红血球
DOI:
10.3791/60659-v
•
09:15 min
•
January 21, 2020
•
Parnian Bigdelou
,
Amir M. Farnoud
2
1
Biomedical Engineering Program
,
Ohio University
,
2
Department of Chemical and Biomolecular Engineering
,
Ohio University
Chapters
00:04
Title
00:42
Erythrocyte Isolation from Whole Blood
02:19
Treatment of Erythrocytes with Ionomycin and Measurement of Hemolysis
04:43
Annexin-V Binding Assay
05:48
Flow Cytometry
06:47
Results: Flow Cytometry and Confocal Fluorescence Microscopy
08:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提供了一种诱导红斑病的方案,即红细胞中程序化细胞死亡,使用碘化钙,碘霉素。通过监测膜外传单中的局部磷脂酰丝氨酸来评估成功的红斑狼疮。已经审查了影响协议成功的因素,并提供了最佳条件。
Tags
Eryptosis
Red Blood Cells
Calcium Ionophore
Ionomycin
Ringer Solution
Glucose-free Buffer
Hematocrit
Cell Suspension
Centrifugation
Incubation
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