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JoVE Journal
Biochemistry

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Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
 

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

Article doi: 10.3791/61784
February 8th, 2021

Summary February 8th, 2021

We describe a single-molecule approach to antigen-antibody affinity measurements using mass photometry (MP). The MP-based protocol is fast, accurate, uses a very small amount of material, and does not require protein modification.

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