JoVE Journal
Immunology and Infection
Immunology and Infection
This content is Open Access.
使用荧光抗生素探针对细菌耐药性的可视化
The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.
If that doesn't help, please let us know.
Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
An unexpected error occurred. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
Click here for the English version
Article
DOI:
10.3791/60743-v
•
08:23 min
•
March 2nd, 2020
使用荧光抗生素探针对细菌耐药性的可视化
March 2nd, 2020
•
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
荧光标记的抗生素是强大的工具,可用于研究抗菌素耐药性的多个方面。本文介绍了荧光标记抗生素的制备及其在细菌中抗生素耐药性研究中的应用。探针可用于通过分光光度测定法、流式细胞测定和显微镜研究细菌抗性机制(例如,排泄物)。
Tags
免疫学和感染,第157期,抗生素,耐药性,细菌,排泄物,荧光,显微镜Related Videos
PLAYLIST
Pharmaceutical Chemistry Formulation of introductory drug. Generic and Ethical Drugs Pre-formulation: Introduction, influencing factors
Ethical drug development processes and generic drug action sites and interactions
Drugs with them: lipids, enzymes, receptors and nucleic acids solid formulations: tablets - considerations in formulation design, composition, production methods, coating, finished product tests, types of tablets
-
Research • ChemistryMass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
-
Research • BiochemistryLaboratory Scale Production and Purification of a Therapeutic Antibody
-
Research • BioengineeringTransport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study
-
Research • BioengineeringGraphene Coatings for Biomedical Implants
Combinatorial Chemistry Semi-solid formulations: purpose of local use, creams, gels - considerations in formulation design, chemical composition, physicochemical properties, preparation methods - Drug Design: Determining Structure Ratio
-
Research • BiologyA Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection
-
Research • Immunology and InfectionNanomechanics of Drug-target Interactions and Antibacterial Resistance Detection
-
Research • BiologyProtein WISDOM: A Workbench for In silico De novo Design of BioMolecules
Active SAR Functional Groups (Pharmacopoeial Colloids: Difference between Colloidal Solutions, Colloid Types) Stability Emulsions: Types of Emulsions (Selection of HLB Composition and Properties, Milk Calculations The quantitative relationship between the drug's structure and its activity (QSAR) (types of surfactants, composition and properties, stability enhancement methods, substances) - Drug Design Strategies 8 Optimize interactions with the target site
-
Research • BiochemistryIdentification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
-
Research • Immunology and InfectionA Calcium Bioluminescence Assay for Functional Analysis of Mosquito (Aedes aegypti) and Tick (Rhipicephalus microplus) G Protein-coupled Receptors
-
Research • BioengineeringIn Silico Modeling Method for Computational Aquatic Toxicology of Endocrine Disruptors: A Software-Based Approach Using QSAR Toolbox
-
Research • EnvironmentExtraction and Characterization of Surfactants from Atmospheric Aerosols
Liquid Formulations Considerations in the formulation design
Physical and chemical properties
Different types of solutions: aqueous solutions, non-aqueous solutions, solutions for local use
Isotonic solutions for injection
-
Education • Core: BiologyTonicity in Animals
-
Education • Lab BioDiffusion and Osmosis- Concept
-
Research • BiologyMeasuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example
-
Research • BiologyInjection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes
-
Research • BiologySimple and Effective Administration and Visualization of Microparticles in the Circulatory System of Small Fishes Using Kidney Injection
Spray Medications: Composition, Advantages, Types
Packaging Prodrugs and Formulation
Opiate painkillers (nsaids) nonsteroidal anti-bacterial (antibacterial drugs) Need for controlled release drugs: Defined release versus controlled release, drug controlled release types, diffusion-based systems and transdermal systems Design and Synthesis of Drugs Microcapsulation Peptides for Drugs: Microcapsules
-
Research • Immunology and InfectionFacilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish
-
Research • BioengineeringAlternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release
-
Research • MedicineSlow-release Drug Delivery through Elvax 40W to the Rat Retina: Implications for the Treatment of Chronic Conditions
Complex - MIC Roses and Liposomes Conservation, Solvent Evaporation Processes, Coacervation Polymerization in Suspension and Emulsion Methods for Preparing Drugs and Intermediates
-
Research • Immunology and InfectionVisualization of Bacterial Resistance using Fluorescent Antibiotic Probes
-
Research • BioengineeringPreparation, Purification, and Use of Fatty Acid-containing Liposomes
-
Research • BioengineeringDelivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes
Controlled release of drugs Systems for release of drugs in physiological control
-
Research • BioengineeringMagnetic and Thermal-sensitive Poly(N-isopropylacrylamide)-based Microgels for Magnetically Triggered Controlled Release
-
Research • BioengineeringSolid Lipid Nanoparticles (SLNs) for Intracellular Targeting Applications
-
Research • BioengineeringFormulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
Preparation of tablets by the wet granulation method and the examination of the physicochemical properties of the tablets