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Immunology and Infection
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细菌毒素引起的响应,用活细胞荧光显微镜的可视化
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Immunology and Infection
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Immunology and Infection
Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
细菌毒素引起的响应,用活细胞荧光显微镜的可视化
DOI:
10.3791/4227-v
•
14:29 min
•
October 01, 2012
•
Peter A. Keyel
,
Michelle E. Heid
,
Simon C. Watkins
,
Russell D. Salter
1
Department of Immunology
,
University of Pittsburgh School of Medicine
,
2
Department of Cell Biology and Physiology
,
University of Pittsburgh School of Medicine
Chapters
00:05
Title
01:09
Preparation of Streptolysin O
05:22
Cell Lytic Assay
06:44
Micropipette Delivery of Toxin to Cultured Cells
10:24
Representative Purification and Imaging Results
13:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
方法从重组净化胆固醇结合毒素链球菌溶血素O<em> E.大肠杆菌</em>和可视化的生活真核细胞毒素结合进行了阐述。本地化的交付毒素诱导靶细胞,揭示新的毒素生物学方面的快速而复杂的变化。
Tags
Visualization
Bacterial Toxin
Live Cell Fluorescence Microscopy
Membrane
Pores
Leakage
Cellular Contents
Influx
Toxin Exposure
Cell Type
Inflammatory Responses
Immune Cells
Macrophages
Pro-inflammatory Cytokines
Gram Positive Bacteria
Virulence Mechanisms
Morphologic Changes
Plasma Membrane
Cholesterol-enriched Surface Protrusions
Extracellular Space
Cellular Defense Mechanism
EM (electron Microscopy)
Chemical Fixation
Immune Cells
Macrophages
Inflammation
Membrane Vesicles
IL-1 Family Cytokines
Article
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