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Immunology and Infection
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小鼠巨噬体化学术的延时成像
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Time-lapse Imaging of Mouse Macrophage Chemotaxis
Please note that all translations are automatically generated.
Click here for the English version.
小鼠巨噬体化学术的延时成像
DOI:
10.3791/60750-v
•
09:33 min
•
April 02, 2020
•
Esther van den Bos
,
Stefan Walbaum
,
Markus Horsthemke
,
Anne C. Bachg
,
Peter J. Hanley
1
Institut für Molekulare Zellbiologie
Chapters
00:04
Introduction
01:04
Prefilling of Chemotaxis Slides
02:25
Isolation of Mouse Resident Peritoneal Macrophages
03:40
Seeding Peritoneal Cells and Adding Chemoattractant
07:31
Results: Chemotaxis of Mouse Macrophages
09:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了使用延时,相位对比显微镜成像小鼠常驻腹腔巨噬细胞在化学策略补充C5a梯度的方法。协议可以扩展到其他免疫细胞。
Tags
Time-lapse Imaging
Mouse Macrophage
Chemotaxis
Inflammatory Diseases
Macrophage Migration
Cell Motility
Chemotaxis Assay
Macrophage Morphology
Cell Velocity
Chemotactic Efficiency
Macrophage Isolation
Peritoneal Lavage
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