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JoVE Journal Bioengineering
Karakterisere enkeltmolekylkonformasjonsendringer under skjærstrøm med fluorescensmikroskopi
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Karakterisere enkeltmolekylkonformasjonsendringer under skjærstrøm med fluorescensmikroskopi

Article DOI: 10.3791/60784-v • 08:47 min • January 25th, 2020
January 25th, 2020 •
1, *2, *3, 3, 1,2, 1,4
1Department of Bioengineering, Lehigh University, 2Department of Materials Science and Engineering, Lehigh University, 3Department of Chemistry, Lehigh University, 4Department of Mechanical Engineering and Mechanics, Lehigh University
* These authors contributed equally

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Vi presenterer en protokoll for å immobilisere enkeltmakromolekyler i mikrofluidiske enheter og kvantifisere endringer i deres konformasjoner under skjærstrøm. Denne protokollen er nyttig for å karakterisere biomekaniske og funksjonelle egenskaper av biomolekyler som proteiner og DNA i et strømningsmiljø.

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Bioengineering Utgave 155 von Willebrand faktor lambda DNA microfluidics skjær flyt TIRF konfokal fluorescens mikroskopi
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