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定量微模式细胞细胞外细胞异细胞的时态参数
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Biology
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JoVE Journal
Biology
Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells
Please note that all translations are automatically generated.
Click here for the English version.
定量微模式细胞细胞外细胞异细胞的时态参数
DOI:
10.3791/60801-v
•
10:21 min
•
September 16, 2020
•
Hugo Lachuer
2,3
,
Pallavi Mathur
2,3
,
Kevin Bleakley
,
Kristine Schauer
2,3
1
Unité Mixte de Recherche 144 CNRS, Molecular Mechanisms of Intracellular Transport group
,
Institut Curie, 75005 Paris, France
,
2
PSL Research University, Paris, France
,
3
Sorbonne Université, Paris, France
,
4
INRIA, Université Paris-Sud, PSL
Chapters
00:04
Introduction
01:11
Micropattern Preparation
03:20
Cell Seeding
04:37
Exocytosis Imaging
05:16
Exocytosis Coordinate Acquisition
06:56
Single Cell Spatial Analysis
07:34
Results: Representative Lysosomal Exocytosis and Spatial Analyses
09:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在微模型细胞上对细胞外细胞的活成像允许这个过程的空间定量。使用微模式进行形态学规范化是揭示细胞过程空间分布的一般规则的杰出工具。
Tags
Micropatterned Cells
Exocytosis
Live Imaging
TOF Microscopy
Cell Secretion
Micropattern Surfaces
Cell Division
Secretory Events
Immune Regulation
Cancer
Proteolytic Enzymes
Invasion
Antigen Presentation
Polylysine Graft Polyethylene Glycol
Quartz Photo Mask
Deep UV Light
Water Droplets
Micropattern Imprints
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