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Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry
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1Oncogeriatric Coordination Unit,Reims University Hospitals, Maison Blanche Hospital, 2Faculty of Medicine,University of Reims Champagne-Ardenne, 3URCACyt, Flow cytometry technical platform,University of Reims Champagne-Ardenne, 4Department of Immunology,Reims University Hospitals, Robert Debre Hospital, 5Department of Internal Medicine and Geriatrics,Reims University Hospitals, Maison Blanche Hospital, 6Faculty of Medicine,University of Reims Champagne-Ardenne
Chapters
- 00:04Introduction
- 01:15Erythrocyte Washing and Dilution
- 02:16Erythrocyte Immunostaining and Fixation
- 03:46Flow Cytometry Analysis
- 05:47Results: Representative Flow Cytometric anti-CR1 Immunostaining Analyses
- 06:27Conclusion
The aim of this method is to determine the CR1 density in the erythrocytes of any subject by comparing with three subjects whose erythrocyte CR1 density is known. The method uses flow cytometry after immunostaining of the subjects' erythrocytes by an anti-CR1 monoclonal antibody coupled to an amplified system using phycoerythrin (PE).
