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1Oncogeriatric Coordination Unit,Reims University Hospitals, Maison Blanche Hospital, 2Faculty of Medicine,University of Reims Champagne-Ardenne, 3URCACyt, Flow cytometry technical platform,University of Reims Champagne-Ardenne, 4Department of Immunology,Reims University Hospitals, Robert Debre Hospital, 5Department of Internal Medicine and Geriatrics,Reims University Hospitals, Maison Blanche Hospital, 6Faculty of Medicine,University of Reims Champagne-Ardenne
Chapters
- 00:04Introduction
- 01:15Erythrocyte Washing and Dilution
- 02:16Erythrocyte Immunostaining and Fixation
- 03:46Flow Cytometry Analysis
- 05:47Results: Representative Flow Cytometric anti-CR1 Immunostaining Analyses
- 06:27Conclusion
Formålet med denne metode er at bestemme CR1-tætheden i erythrocytter af ethvert forsøgsperson ved at sammenligne med tre forsøgspersoner, hvis erythrocyt CR1-tæthed er kendt. Metoden anvender flowcytometri efter immunfarvning af forsøgspersonernes erythrocytter med et anti-CR1 monoklonalt antistof koblet til et forstærket system ved hjælp af phycoerythrin (PE).
