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Journal / Biochemistry / Visualización de receptores de estrógeno en…
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Biochemistry

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JoVE Journal Biochemistry
Visualización de receptores de estrógeno en colones de ratones con enfermedad de Crohn inducida por TNBS utilizando inmunofluorescencia
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Visualización de receptores de estrógeno en colones de ratones con enfermedad de Crohn inducida por TNBS utilizando inmunofluorescencia

Article DOI: 10.3791/60813-v • 08:02 min • March 12th, 2020
March 12th, 2020 •
1, 2, 3, 2, 1
1Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, 2Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, 3Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz

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El protocolo presenta un modelo murino completo validado inducido por TNBS de la enfermedad de Crohn y métodos para la visualización de receptores de estrógeno por inmunohistoquímica utilizando inmunofluorescencia de secciones de colon fijas en formalina incrustadas en parafina.

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Bioquímica Número 157 Enfermedad de Crohn modelo murino receptores de estrógeno receptor de estrógeno acoplado a la proteína G GPER ER ER inmunohistoquímica microscopía confocal
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