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在小鼠的文室内使用光遗传学的两种不同的实时位置偏好范式
JoVE Journal
Behavior
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JoVE Journal
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Two Different Real-Time Place Preference Paradigms Using Optogenetics within the Ventral Tegmental Area of the Mouse
Please note that all translations are automatically generated.
Click here for the English version.
在小鼠的文室内使用光遗传学的两种不同的实时位置偏好范式
DOI:
10.3791/60867-v
•
05:50 min
•
February 12, 2020
•
Zisis Bimpisidis
,
Niclas König
,
Åsa Wallén-Mackenzie
1
Department of Organismal Biology, Unit of Comparative Physiology
,
Uppsala University
Chapters
00:04
Introduction
00:36
Setting up the Control of the Laser Source
01:44
Setting Up the Experiment for the RT-PP (Real-Time Place Preference) Approach and NCP (Neutral Compartment Preference) Approach
03:13
Performing an Experiment Using Laser Stimulation
04:24
Results: Real-Time Place Preference Experiment
05:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了两个易于遵循的分步协议,用于在小鼠中使用光遗传学的放置偏好范例。使用这两种不同的设置,偏好和回避行为可以在同一设备中以高空间和时间选择性,以简单明了的方式进行可靠的评估。
Tags
Optogenetics
Ventral Tegmental Area
Real-time Place Preference
Reward
Aversion
Microcontroller
Laser
Optic Fiber
Arena Setup
Tracking Software
TTL Signal
Neutral Compartment Preference
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