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Developmental Biology

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JoVE Journal Developmental Biology
Microwaving og Fluorophore-Tyramid for Multiplex Immunfarvning på Mouse Adrenals − Brug af ukonjugerede primære antistoffer fra samme værtsart
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Microwaving og Fluorophore-Tyramid for Multiplex Immunfarvning på Mouse Adrenals − Brug af ukonjugerede primære antistoffer fra samme værtsart

Article DOI: 10.3791/60868-v • 07:37 min • February 21st, 2020
February 21st, 2020 •
1,2, 2, 2, 2
1College of Animal Science & Technology, Henan University of Science and Technology, 2Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University

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Der er etableret flere forskellige metoder til multipleximmunfarvning ved hjælp af primære antistoffer fra samme værtsart. Her beskriver vi brugen af mikrobølgemedieret antistofstripning og fluorophore-tyramid til at blokere antistofkrydsreaktivitet under multipleximmunfarvning på formalin-fast paraffin-indlejrede musebinyresektioner.

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Udviklingsbiologi multiplex immunfarvning immunfluorescens tyramid microwaving antigen hentning antistof stripning
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