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Journal
/
Biochemistry
/
在Vivo羟基基蛋白足迹研究
卡诺哈布迪特
人的蛋白质相互作用
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in
Caenorhabditis elegans
Please note that all translations are automatically generated.
Click here for the English version.
在Vivo羟基基蛋白足迹研究
卡诺哈布迪特
人的蛋白质相互作用
DOI:
10.3791/60910-v
•
08:00 min
•
April 01, 2020
•
Jessica A. Espino
,
Lisa M. Jones
1
Department of Pharmaceutical Sciences
,
University of Maryland
Chapters
00:04
Introduction
00:30
Microfluidic Flow System Assembly
02:05
Microfluidic Flow System for in vivo FPOP
04:13
In vivo FPOP
05:52
Results: IV-FPOP Oxidative Modification of Proteins within
C. elegans
07:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
体内蛋白质快速光化学氧化(IV-FPOP)是一种羟基基基蛋白足迹技术,能够绘制其原生环境中的蛋白质结构图谱。该协议描述了 IV-FPOP 微流体流系统的组装和设置。
Tags
In Vivo
FPOP
Protein-protein Interactions
Protein Structure
Caenorhabditis Elegans
Mass Spectrometry
Flow System Assembly
Fluorinated Ethylene Propylene
Fused Silica
Epoxy Resin
Outlet Capillary
Magnetic Stirrers
M9 Buffer
Three-way Valve
Syringe Pump
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