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Journal / Bioengineering / Trækkraft kraft mikroskopi til undersøgelse B…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Trækkraft kraft mikroskopi til undersøgelse B Lymfocyt Aktivering
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Trækkraft kraft mikroskopi til undersøgelse B Lymfocyt Aktivering

Article DOI: 10.3791/60947-v • 09:28 min • July 23rd, 2020
July 23rd, 2020 •
1, 1, 1, 2, 1
1Institut Curie, PSL Research University, INSERM U932, 2Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier (Grenoble 1)

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Her præsenterer vi en protokol, der anvendes til at udføre trækkraft mikroskopi eksperimenter på B-celler. Vi beskriver fremstillingen af bløde polyacrylamid geler og deres funktionalisering, samt dataindsamling på mikroskop og et resumé af dataanalyse.

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Bioengineering Bioengineering bløde geler trækkraft antigen biofysik immunologi B-celler
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