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毛利精密切肝片作为肝脏生物学的Ex Vivo模型
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Medicine
Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology
Please note that all translations are automatically generated.
Click here for the English version.
毛利精密切肝片作为肝脏生物学的Ex Vivo模型
DOI:
10.3791/60992-v
•
12:36 min
•
March 14, 2020
•
Michael A. Pearen*
1
,
Hong Kiat Lim*
1
,
Francis D. Gratte
3
,
Manuel A. Fernandez-Rojo
4,5
,
Sujeevi K. Nawaratna
,
Geoffrey N. Gobert
,
John K. Olynyk
9
,
Janina E. E. Tirnitz-Parker
10
,
Grant A. Ramm
4
1
Hepatic Fibrosis Group
,
QIMR Berghofer Medical Research Institute
,
2
School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute
,
Curtin University
,
3
School of Veterinary and Life Sciences
,
Murdoch University
,
4
School of Medicine
,
The University of Queensland
,
5
Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC
,
6
School of Medicine
,
Griffith University
,
7
School of Biological Sciences
,
Queen’s University Belfast
,
8
Department of Gastroenterology & Hepatology
,
Fiona Stanley and Fremantle Hospital Group
,
9
School of Medical and Health Sciences
,
Edith Cowan University
,
10
Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences
,
University of Western Australia
Chapters
00:00
Introduction
01:23
Setup of the Vibratome
03:15
Liver Removal and Preparation
06:07
Production of Liver Slices
10:14
Results: Viability, Collagen, and Effects of Selected Bile Acids
11:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议为从小鼠中生产可行的精确切肝片提供了一种简单可靠的方法。前活体组织样本可在实验室组织培养条件下维持多日,为检查肝病理生物学提供了灵活的模型。
Tags
Precision-cut Liver Slices
Ex Vivo Model
Liver Biology
Vibrating Blade
Bile Acids
Cholestatic Liver Injury
Hepatic Fibrogenesis
Krebs-Henseleit Buffer
Peltier Thermoelectric Cooler
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