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Journal
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Developmental Biology
/
植物组织细胞壁中阿拉比诺格拉坦蛋白和皮克廷的荧光免疫化
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
Please note that all translations are automatically generated.
Click here for the English version.
植物组织细胞壁中阿拉比诺格拉坦蛋白和皮克廷的荧光免疫化
DOI:
10.3791/61034-v
•
10:14 min
•
February 27, 2021
•
Mário Costa
2
,
Ana Marta Pereira
,
Sílvia Coimbra
2
1
Departamento de Biologia
,
Faculdade de Ciências da Universidade do Porto
,
2
Laboratório Associado para a Química Verde
,
3
Dipartimento di Bioscienze
,
Universitá degli Studi di Milano
Chapters
00:09
Introduction
00:39
Sample Fixation and Dehydration
02:17
LR-White Resin Embedding
03:26
Sample Trimming and Sectioning
05:12
Immunolocalization
07:31
Results: Subcellular Localization of Proteins and Pectins
09:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议详细描述了用于阿拉伯甘草蛋白和果胶免疫的植物材料是如何固定的,嵌入亲水丙烯酸树脂中,在玻璃滑梯上分节并安装。我们显示细胞壁相关的表位将被检测到与特定的抗体。
Tags
Fluorescent Immunolocalization
Arabinogalactan Proteins
Pectins
Cell Wall
Fixation
Vacuum
Dehydration
Embedding
LR-white Resin
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