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Fluorescerende immunolocalisering af Arabinogalactan proteiner og pektiner i cellevæggen af plantevæv
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
DOI:

10:14 min

February 27, 2021

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Chapters

  • 00:09Introduction
  • 00:39Sample Fixation and Dehydration
  • 02:17LR-White Resin Embedding
  • 03:26Sample Trimming and Sectioning
  • 05:12Immunolocalization
  • 07:31Results: Subcellular Localization of Proteins and Pectins
  • 09:32Conclusion

Summary

Automatic Translation

Denne protokol beskriver i detaljer, hvordan plantematerialet til immunolocalisering af Arabinogalactan proteiner og pektiner er fastgjort, indlejret i en hydrofil akrylharpiks, sektionsopdelt og monteret på glasrutsjebaner. Vi viser cellevæg relaterede epitoper vil blive opdaget med specifikke antistoffer.

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